The analytic protocol used for the Project was a modification of this methodology.
A modification of this methodology was used to localize sites of superoxide production in rat endothelium in situ (Babbs et al. 1991a, b).
The urea content in corresponding serum samples was determined using the same kit without modification of the methodology as specified by the manufacturer.
We carried out a modification of SAGE methodology (microSAGE), useful for the analysis of limited quantities of tissue samples, on normal human cervical tissue obtained from a donor without histopathological lesions.
Minor modification of a methodology developed by Gibson and co-workers, which allows the substitution of different aryl groups in the 1- and 8-positions of a carbazole ring, gave 1,8-Xyl2-3,6 tBu2candH (L H) and 1,8-Mes2-3,6 tBu2carbH (L H),[ 20] thus facilitating convenient tuning of the steric properties of the ligand.
Suitable modification of the methodologies needs to be further studied to adjust for such informative censoring mechanisms related to terminal events in the recurrent event analysis [ 26].
Modification of conventional methodologies to include filtration of cell suspensions, use of "proliferation control" and "cytotoxicity control" cultures as well as vital staining were found to be essential for the valid assessment of primary soft agar cultures in our laboratory.
In any case, we will consider any suggested modifications of our methodology.
Osteoclast formation was determined using the specific osteoclastic marker TRAP using a modification of Burstone's methodology (Burstone 1958).
We then introduce an uncertainty assessment framework based on the integration of the notion of the "cascade of uncertainty" (e.g. Schneider 1983; Wilby and Dessai 2010) and a modification of the NUSAP Numeral Unit Spread Assessment Pedigreee) methodology (Funtowicz and Ravetz 1990; van der Sluijs et al. 2005a, b).
