In this paper, we focus on the possibility to improve coexistence performance of WiFi and ZigBee networks by exploiting novel programmable architectures of wireless devices able to support run-time modifications of medium access operations.
HEK-swAPP cells (gift from Dr. C. Haass) were grown in Dulbecco's modification of Eagles medium (DMEM /F12 medium supplemented with 10% foetal bovine serum (FBS) (Gibco BRL, Rockville, MD, USA), 1% penicillin and 1% streptomycin.
Generally, strain selection or improvement by genetic modification, optimization of medium formulation, and fermentation conditions are the main options to improve enzyme activity and reduce production cost [ 5, 6, 10, 15].
All cells were maintained in Dulbecco's modification of Eagles medium (DMEM) with five percent fetal bovine serum and one percent L-glutamine.
Future studies will also investigate modification of the medium (which was designed for proliferation and compatibility with a biphasic poxvirus production process [ 25]) to increase not only virus titers and productivity but also the critical CCI.
ES cells were cultured without feeders in the presence of leukemia inhibitory factor (LIF) either in Glasgow modification of Eagles medium (GMEM) containing 10% fetal calf serum or in serum-free N2B27 supplemented with MEK inhibitor PD0325901 (1 μM) and GSK3 inhibitor CH99021 (3 μM), together known as 2i (Ying et al., 2008).
The viruses or samples were diluted 1 10 to a final concentration of 1 1 000 000 in a separate V-bottom 96-well plate with serum-free Dulbecco's modification of Eagle's medium medium.
The GYS medium is a modification of the mineral medium described by Buckland [ 26] that consisted of 10 g/l glycerol, 20 g/l yeast extract, 2 g/l (NH4 2SO4, 2 g/l K2HPO4, 0.01 g/l CaCl2.2H2O, 0.01 g/l FeSO4.7H2O, 0.1 g/l MgSO4.7H2O.
These coordination solutions require a customized tuning of the MAC parameters used in the multi-technology interfering networks or even modifications of the medium access operations.
