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In contrast to the logistic limitations of the International Space Station ISS and other space-based research platforms, parabolic flights provide frequent and repeated access to microgravity and therefore allow replication and modification of experiments within a reasonable time frame, which are not only characteristics, but rather requirements, of modern biomedical research.
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But, "after several months and the x-th modification of the experiment, I ran out of ideas … [on] which experimental artifact could be responsible for this.
Protocol modifications of sequencing experiments could modestly improve performance, such as the use of whole genome amplification [49], [50], but this can introduce loss of heterozygosity.
But one theme all members could agree on was how to decrease the suffering of animals with better technical solutions, which is also reflected in the requested technical modifications of the experiment that was often communicated from the committee to the researcher.
In the next series of experiments, modifications were made to the protocol in order to 1) decrease animal to animal variation, 2) limit the nonspecific toxic effects of bolus DT-treatment and 3) better model a "western" diet.
ANSYS CFX gave similar results with that of experiments by modification of the cross viscosity model.
The authors are requested to address (by inclusion of experiments or appropriate modification of the text) the following points in a revised manuscript: 1) Figure 1.
However, as the goal of RNA modification mapping experiments is to place modified nucleosides in the correct RNA sequence context, RNase digestion products that do not contain a modified nucleoside can be considered to be information-deficient oligonucleotides.
The comparison is done by means of receiver operating characteristic curves on two datasets: a panel of 11 human tissues with confirmed alternative splicing events; and a modification of a spike-in experiment where 25 transcripts were hybridized to HeLa cells [ 42, 43].
We are also interested in verifying the outcome of experiments that include modifications of the biological model.
The cluster also includes some irrelevant experiments (e.g. treatment of astrocytes with tetrahydrocannabinol), but there is a common overall theme of experiments involving genetic modifications and/or drug treatments of model organisms (mostly mouse).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com