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It neither involves any functionalization of supports [27] nor does it require prior modification of enzymes [41].
The most effective chemical modification of enzymes is achieved by using reagents such as activated polyethylene glycol or dinitrofluorobenzene that add hydrophobic groups.
Meanwhile, an increasing number of computational methods have been developed to assist in the modification of enzymes during the past few decades.
The reported approach shows the glucose sensing capabilities of a system that merges the characteristics of bioelectronics fabricated using redox-containing polymer networks with those based on the surface modification of enzymes with electron relays.
Pathway fluxes can be regulated by feedback inhibition, allosteric interactions and post-translational modification of enzymes.
Modification of enzymes to improve catalytic properties has been an objective for the last 30 years.
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The study also opens a new avenue for modification of enzyme activity and stability that offers great promise in applications such as biological catalysis, biosensing and enzyme engineering.
The impact of directed evolution and site-specific mutagenesis on the industrial utility of enzymatic catalysis through the modification of enzyme structure and function is clearly an important area of research in bioprocess engineering.
The modification of the multiple metabolites involved in TCA cycle may have occurred due to the modification of enzyme activities of TCA cycle.
This could occur through oxidative modification of amino acids or irreversible modification of enzyme function by electrophiles (Shinyashiki et al. 2008).
Meanwhile, the modification of enzyme encoded genes that are involved in phenylpropanoid pathway could promote the biosynthesis of isoflavone [ 31, 35].
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