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Following the injection, in vivo electroporation was used according to the modification of described elsewhere [50].
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In order to speed up the computations we propose several modifications of described algorithm that make use of input data structure given in Section 2.2.1 and take into account algorithm specifics.
The method that we describe is a modification of that described by Massey [ 31].
The DC culture protocol was described previously (Castellino et al. 2000) and is a modification of that described by Inaba et al. (1993).
BR and FDAH were measured with a modification of method described by Pane et al. [44].
In the second experiment, the muscle specimen was prepared through a modification of previously described methods [15, 16].
Quantitative RT-PCR were carried out with minor modification of previously described procedures [9].
Anchorage-independent growth was determined using a modification of previously described methods [40].
ppGpp was detected using a slight modification of previously described procedures [27], [28].
The method used was a modification of that described by Towbin et al. [54].
Secreted virulence factors were prepared by using a modification of previously described methods [29].
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