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The modification methodology was developed in our previous study [22], including the successive three-step process: plasma pretreatment of PTFE, graft polymerization of AA onto plasma-treated PTFE to form PAA/PTFE, and TiO2 self-assembly onto the PAA/PTFE to obtain the composite membranes.
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In the second protocol modification, a methodology was developed for high-throughput solution-based TGE using automation equipment at a large genome center [ 10], however this protocol is not amenable to implementation in small- or medium-sized laboratories.
A modification of this methodology was used to localize sites of superoxide production in rat endothelium in situ (Babbs et al. 1991a, b).
All modifications to methodology were discussed and approved by the CIFA Steering Committee and CCG.
Nitroxidation was the major cysteome modification in all cases, and the d-SSwitch methodology was capable of identifying and quantifying modification of specific cysteine residues.
To analyze the effect of NTD expression on UV survival, similar methodology was used with minor modification.
The methodology was that as previously published with the modification as a C-terminal amide.
PCR methodology was performed as previously described with further modifications [ 8].
Methodology was based on Meyer et al. [ 23], with modifications by K. Mockaitis as described by Schwartz et al. [ 22].
Standard methodology was used.
Their methodology was roundly criticized.
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