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The histone modification levels and recruitments of transcription factors were examined by ChIP assays as described previously45 with some modifications.
Our findings confound existing views and point to active cellular mechanisms which dynamically regulate histone modification levels and account for exon-intron marking.
Thus, we attributed our results as indicative of a complex bona fide marking system of exon-intron structures defined by histone modification levels and not by nucleosome distributions, as reported previously [19], [20], [21], [22], [23].
Studies have shown the strong association between histone modification levels and gene expression levels.
Is there an association between specific histone modification levels and gene sizes, or gene expression levels?
Intuitively, this corrects for differences in the dynamic range of changes to histone modification levels and for differences in segment size.
Similar(49)
The transcriptome bias toward high GCA parents in F1 hybrids attributed to H3K36me3 modification both on global modification level and specific signaling pathways.
Hence, the correct approach for assessing correlation between serum AGE levels and RA activity would be to assess the correlation between a specific protein modification level and a synovitis, present in all joints in the body during the interval of this protein's half-life.
Based on the original gene annotation tables from [ 25- 27], an in-house Perl script file was used to map nucleosome occupancy and histone modification levels to gene and the corresponding promoter region, in which if multiple probes are assigned to the same gene or promoter region then we use their mean value.
Using the MeshLab [8] tool, we applied random vertex displacement filter by varying the maximum displacement parameter (The parameter was set as 0.1, 0.2, and 0.3 for modification levels 1, 2, and 3, respectively).
Modification levels are, by and large, uniform across tissues coming from the same individual animal.
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