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We found that Rpb1 is also sumoylated in yeast cells upon UV radiation or impairment of transcription elongation, and this modification is independent of DNA damage checkpoint activation.
UV-induced Rpb1 sumoylation was slightly higher in mec1 sml1 cells than in the isogenic wild type and sml1 cells (Fig. 1C), indicating that this covalent modification is independent of the checkpoint activation.
Thus, the Pex5p modification is independent of the Lys and Lys.
This promising procedure modification is independent of different cephalomedullary implant manufacturers and specific implant designs, but needs to be evaluated in further clinical settings.
Interestingly, we found that WT1 depletion had no influence on m6A abundance, suggesting that WTAP's role in regulating m6A modification is independent of its previously described binding partner WT1.
Furthermore, in the same study, ZCCHC6 enzyme was found to be responsible for the monouridylation of group II let-7 pre-miRNAs and this modification is independent of the Lin28 protein but is critical for the production of this particular miRNA [ 96].
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Modification of graft function was observed with both creatinine measurement and estimation of the glomerular filtration rate (using abbreviated modification of diet in renal diseases (aMDRD) formula), suggesting that graft function modification was independent of biases potentially associated to aMDRD formula.
This indicates that the coexpression driven by co-modification is independent of co-function.
Our demonstration that Salmonella-mediated cytoskeletal modifications are independent of inositolphosphates will focus future studies on elucidating alternate pathogenic consequences of InsP5 metabolism, including ion channel conductance and apoptosis.
In addition, our results in cultured cells revealed that USP25m was phosphorylated (in Tyr and Ser/Thr residues) and acetylated, and that these modifications were independent of USP25m catalytic activity, as the wild-type protein and the inactive mutant were similarly modified (Figure S3).
These post-translational modifications were independent of the phospho-cysteine chemical reaction and are consistent with frequently observed modifications on recombinant proteins expressed in E. coli, particularly within N-terminal hexa-histidine tag regions, but also on lysine residues.
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