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This modification is followed by proteolytic cleavage within the CAAX motif and carboxymethylation of the exposed cysteine residue.
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The reactions involved in this bitumen modification were followed by using different techniques, such as thin layer chromatography (TLC FID), and Fourier transform infrared spectroscopy (FTIR).
Polymer modification was followed by tetraethoxysilane hydrolysis and condensation reactions.
Briefly, precursor peptide modification was followed by SDS-PAGE and ESI-MS.
A 15-min equilibration period between each modification was followed by measurements of hemodynamics, arterial blood gases and respiratory parameters.
Patrick et al. [ 28] protocol with slight modification was followed to study the antioxidant potential of SCEE.
Saeed et al. [ 25] methodology with slight modification was followed for ABTS (2,2 azobis, 3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activity.
Ahmad et al. [ 13] methodology with slight modification was followed for ABTS (2, 2 azobis, 3-ethylbenzothiozoline-6-sulphonic acid) radical scavenging activity.
Hydroxyl scavenging activity % = 1 ‒ Asorbance of sample / Absorbance of control × 100 Re et al. [ 21] methodology with slight modification was followed for ABTS (2, 2 azobis, 3-ethylbenzothiozoline-6-sulphonic acid) radical cation scavenging activity.
Transfected cells were washed with Tyrode´s buffer (135 mM NaCl, 10 mM KCl, 10 mM MgCl2, 1mM CaCl2, 10 mM HEPES pH 7.2, 0.1% BSA) and incubated with 0.2 or 2.0 μM ACP-S and 1 μM CoA derivatives for 30 min at room temperature and finally washed four times with Tyrode´s CoA-biotin modification was followed by a labeling step with 1 nM SA-atto550 for 15 min at room temperature.
Structural modifications were followed by Raman microspectrometry.
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