The modification was completed after a certain time period.
The modification was completed by attachment of NB via Schiff base formation between the NB and immobilized Glu.
Modification was completed by NaOH (final concentration, 0.3 M) treatment for 5 min at room temperature, followed by ethanol precipitation.
DNA modification was completed by incubation in 5 µL NaOH 3N for 15 min at room temperature.
Modification was completed by NaOH treatment (final concentration 0.3 M) for 5 min at room temperature, followed by ethanol precipitation.
Modification was completed by the treatment of 5.5ul 3 M NaOH (final concentration 0.3 M) for 15-20 minutes at room temperature.
Modification was completed by 0.3 M NaOH treatment for 5 min at room temperature, followed by ethanol precipitation, DNA was resuspended in distilled water and stored at -20°C.
Modification was completed by NaOH treatment (final concentration 0.3 M) for 5 min at room temperature, followed by addition of 24 μL of sodium acetate 3 M, pH 5.2, followed by ethanol precipitation.
DNA was purified with the Wizard DNA Clean-UP System (Promega. Madison, WI, USA), and DNA modification was completed by the addition of 5.0 μl of NaOH 3 M at room temperature for 15 min. Precipitation was carried out through the addition of 30 μl of ammonium acetate 5 M (pH 7.0), 350 μl of ethanol and 1 μl of glycogen (20 μg/uL) (Invitrogen Life Technologies, Carlsbad, CA, USA).
When her modifications were complete, she used her Ford F-150 pickup truck and a 16-foot trailer to transport the car, along with two assistants and her 2-year-old son, to the show in New Mexico and then the one in California.
