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Cleanup protocol was modified to preserve microRNA (modification instructions provided by Exiqon).
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While children received behavior modification instruction, parents/caregivers attended their own support class in which elements of solution-focused brief therapy were used by a psychologist or dietitian.
Prior to randomisation, participants completed a 2-week run-in period, in which participants received lifestyle modification instruction over the course of six laboratory visits.
In a pilot study, the questionnaire was tested on ten respondents, which led to slight modifications regarding instructions and clarity of questions.
In all study arms, after completing the baseline measurements, participants will receive instruction in lifestyle modification, including individualized instruction on weight loss, exercise, and diet.
Recommendations for modifications to instruction call for increased emphasis on inquiry-based activities (Metz 2004; NRC 2000; NSTA 2002) and opportunities for scientific modeling (Lehrer and Schauble 2004).
The survey was piloted for approximately 1 month and after minor modifications, for instruction clarity and reduced length, distributed via email to attending physicians, nurse practitioners and residents training in the fields of internal medicine (IM) and emergency medicine (EM).
In the first phase, peer educators spent 40 h learning the education curriculum, behavior modification techniques, group instruction and mediation methods, and employee standards.
This modification deleted some instructions from the original 10NES program.
Other than the modification to the instructions to try to elicit more noun responses, the experiment was identical to Experiment 4A.
Participants also received advice on behavioral modification, including written instructions, to increase physical activity (to walking for at least 30 min most days of the week).
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