Variation in ammonemia levels was associated with a modification in volumes and SG (p < 0.05).
Thus, brainstem and cerebellum volumes were not correlated with ammonemia levels, and it variation was not associated with modification in volumes in these regions.
Moreover, Ghezzi et al. [ 14] over a follow-up of 3 years, did not register any major modifications in volume reduction or symptom improvement.
These reversible effects were probably associated to osmotic stimuli resulting from modifications in the volume and distribution of extracellular fluids and plasma during flight and landing.
Literature well documents the extent to which the distribution of ventilation becomes progressively more inhomogeneous at high lung volume [ 32] or under the effect of modifications in tidal volumes, flows, posture and in the presence of asymmetrical geometry of lung units as well as in normal lungs [ 31, 33].
BigDye Terminator V3.1 Cycle Sequencing Kit and SNaPshot Multiplex Kit (Applied Biosystems, Foster City, CA) were used to validate SNPs, following the manufacturer's protocols with some modifications in reaction volumes and/or quantity of proprietary reagents.
Modifications included an increase in volume of buffer AP1 (from 400 to 600 μL), buffer AP2 (from 130 to 200 μL), and RNase A (from 4 to 6 μL), as well as a longer incubation time with buffer AP1 (at 15 min) for cell lysis.
The PCR amplification and genotyping were done according to le Flèche et al. [ 17] with only a modification in the total reaction volume to 30 μL.
In healthy volunteers, injection of contrast material did not produce any detectable modification in the lung tissue volume calculated using Lungview®, probably because the contrast material remained strictly intravascular in the presence of an intact alveolar capillary barrier and was rapidly eliminated in the urine [ 2].
The principal methods that an IDE exerts on a dominated nation are the modification in the investment or trade volume, leading to an improvement or deterioration of dominated nation's commercial balance.
