Sentence examples for modification in the locus from inspiring English sources
To exclude the latter hypothesis and to check the accessibility for recombination of the PfI2 locus, we introduced a targeted modification in the locus without loss-of-function.
We were not able to find a meaningful base pair modification in the CG11188 locus for the second allele nutmeg 21.3.
Splinter, E. et al. CTCF mediates long-range chromatin looping and local histone modification in the beta-globin locus.
Clones of transformed, neomycin resistant ES cells were screened for the insertion of the correct modification in the hmmr gene locus.
NAKA fails to complement armor plate changes when crossed with a typical Canadian low-plated population (Schluter et al., 2004), suggesting that NAKA and other low-plated fish share a modification in the same major locus.
This is consistent with hyperexpression of Hoxa9 in AR1 cells in which the repressive histone modifications in the Hoxa9 locus markedly reduced (Fig. 1C, 1E and 2B).
Ablation of menin abrogates Cdx4 access to the chromatin target and significantly reduces both active and repressive histone H3 modifications in the Hoxa9 locus.
We further demonstrated that menin is a crucial regulator for both active and repressive histone 3 modifications in the Hoxa9 locus.
We showed that histone modifications, especially methylation of histone H3, play an important role in determining the level of Hoxa9 expression, and menin is crucial for both active and repressive histone modifications in the Hoxa9 locus.
Having identified the potentially active and repressive element, we used quantitative chromatin immunoprecipitation (qChIP) to map a variety of histone modifications in the Hoxa9 locus, as represented by amplicons 1 4 (Fig. 1A) in three different cell lines, each expressing a distinct level of Hoxa9 (Fig. 1E).
The CCR5 megaTAL nuclease established resistance to HIV in cell lines and disrupted the expression of CCR5 on primary human CD4+ T cells with a high efficiency, achieving up to 80% modification of the locus in primary cells as measured by molecular analysis.
