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SCNT is a practical way to produce targeted genetic modification in pigs.
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Engineered zinc finger nuclease (ZFN mediated genetic modification in somatic cells combined with somatic cell nuclear transfer (SCNT) technology provides a new approach for targeted modification in pig genome.
Multiple genetic modifications in pigs can essentially benefit research on agriculture, human disease and xenotransplantation.
Using currently available approach, generating specific gene modifications in pigs requires two steps.
The cloning technology provides an opportunity to improve livestock production efficiency and to create genetic modifications in pigs for agriculture and medicine.
Interestingly, amino acid substitutions were discovered in these two modification sites in pig (T264Q and S478T), such substitutions may make this protein more stable for oxidative biotransformation of various endo- and xenobiotics, and further biomedical studies focusing on the CYP3A4 gene (using the pig as a model) should be aware of such modifications.
The present study was designed to further study how COS characteristics: molecular weight (MW) and degree of deacetylation (DD); affect the growth performance, nutrient digestibility, and small intestinal modifications in weaning pigs.
A commercially available enzyme immunoassay (Abbott Laboratories) was used, with some modifications to detect IgG anti-HEV in pigs.
Results indicate that PCR-RFLP technique allowed detecting modifications on the gastrointestinal microbial ecology in pigs fed with different additives, such as increased biodiversity by sodium butyrate in feed.
An example is the discovery in pigs of CYP4A21, an enzyme that catalyzes 6α-hydroxylation of bile acids [ 43], a bile acid modification not found in humans.
In pigs, the number was 16.
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