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DNA methylation is an important epigenetic DNA modification in bacteria.
In general, this is an interesting paper and it offers a testable hypothesis, which can lead to better understanding the mechanisms of post-translational modification in bacteria.
Studies of rRNA modification in bacteria demonstrated that both adenosines are dimethylated in concert (25, 28, 38), indicating that the primer extension assay would give a reliable estimate of the overall dimethylation status at both adenosines.
On the contrary, if these genes are present in distantly related genomes (e.g. genomes from different bacterial phyla), this may point to either an ancient origin of this modification in Bacteria followed by numerous independent secondary losses, or to a recent origin followed by horizontal gene transfer across bacterial phyla.
Slow growing pathogenic mycobacteria such as M.tb modify their mycolic acids by cyclopropanation which is a common membrane modification in bacteria and plants [ 28, 29] and essential for viability, drug resistance and cell wall integrity [ 30] playing an important role in M.tb pathogenesis [ 31, 32].
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Even though protein initiator methionine excision (NME) and N-terminal acetylation (NTA) have been relatively well investigated in eukaryotic proteomes, few studies were dedicated to these modifications in bacteria up to now.
Hence, this example illustrates that HGT can drive major physiological modifications in bacteria.
The kinetic signatures of 4mC and 5caC are sufficiently different to allow for discrimination of the two types of cytosine modifications in bacteria.
We showed that NGS analysis is able to identify such novel gene acquisitions and other genomic modifications in bacteria very efficiently.
Methylation of adenine or cytosine is a part of the restriction modification system in bacteria; by methylating its own DNA by the use of methyltransferases it is possible to separate this DNA from foreign DNA.
Activated by PhoP-PhoQ, the PmrA-PmrB encoded by pmrCAB operon is the major regulator to mediate the LPS modification in Gram-negative bacteria [ 54].
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