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With the method established in this study, more tRNA modification genes in plants should be able to be identified.
In fact, the adjacent restriction and modification genes in many RMSs were found to have remarkably different evolutionary histories.
Using a reverse genetics approach, we successfully isolated and identified five tRNA modification genes in Arabidopsis thaliana.
We conclude that the method established in this study will facilitate the identification of tRNA modification genes in a wide variety of plant species.
Based on their corresponding genes in the cep and bal clusters [ 7, 8], the functions of the modification genes in the vcm cluster were annotated (Table 2).
Both these families cluster with tRNA modification genes in several Archaea and follow the expected phylogenetic distribution (see "Archaeal tRNA modification" subsystem in SEED).
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Three additional essential RNA modification genes were identified in S. aureus, rimM, ymfA and thdF.
Chromatin modification genes play crucial roles in development and disease.
Another form of non-genetic inheritance is the epigenetic modification of genes in the zygotes.
More importantly, we have predicted and summarized the tRNA modification candidate genes in plants.
First, chromosomal deletions preclude easy modification of genes in comparison to plasmid-based expression.
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