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In addition, we show that multi-NLS ZFN proteins attenuate off-target effects and that codelivery of ZFN protein pairs facilitates dual gene modification frequencies of 20 30% in CD4+ T cells.
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As shown in Figure 5A, HeLa cells subjected to three consecutive treatments with 3 μM TAT-TALEN proteins showed a modification frequency of 3% as measured by the Surveyor assay.
Paramagnetic property of dusts, leads to modification of frequencies of dust lattice waves.
To assess if the fetal electrocardiogram especially ST segment is modified by congenital heart diseases: modifications in frequencies of the different ST events and modifications in signal quality.
As a result of this modification, natural frequency of fifth mode shape (50 Hz) of the combine cutting platform is shifted to 48 Hz.
Nevertheless, it was concluded that one of the possible consequences of differential susceptibility to P. vivax malaria could be modifications in allelic frequencies of FY*A and FY*B in populations exposed to P. vivax, the most prevalent malaria species in the Brazilian Amazon region.
Finally, we evaluate whether rates of detection by humans can be used to predict and explain evolutionary modifications of relative frequencies of pygmy grasshopper colour morphs in different habitat types in the wild.
When an axonemal segment bends, this induces the compressed and dilated conformations of the tubulin monomers and, consequently, the modification of the spatial frequencies of the appendages that the outer doublets carry.
This minor modification resulted in high frequencies of peptide-specific splenocytes (Fig. 1f) and moderate but robust in vivo cytotoxicity of Jmut-induced effectors (Fig. 1g).
Selected ZFN pairs were tested for the ability to induce sequence changes in a Cel1 assay and we observed frequencies of genomic modification up to 18.7% at the endogenous hTERT locus.
Frequencies of therapeutic modification based on the results from non-invasive tests and invasive tests were also evaluated across additional diagnostic tests.
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