Moreover, this assay provides modification-free and enzyme-free protein detection without sophisticated instrumentation and might be generally applicable to the detection of other protein targets in the future.
By coupling the cascade two-stage target sequences-recyclable toehold strand-displacement with aptamer-based target recognition successfully, it is the first report on a novel non-label, modification-free and DNA extraction-free ultrasensitive fluorescence biosensor for detecting viable S. enteritidis directly, which can discriminate from dead S. enteritidis.
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The incidence of dose modifications was analysed using Kaplan Meier curves and the ratio of modification-free rates at 12 weeks of treatment were estimated using 95% confidence intervals.
The resulting preparation containing various oxidized lipids was used to determine the modification of free amino groups in horse skeletal muscle apomyoglobin which contains one free N-terminal amino acid and 19 ε-lysyl amino group.
Using the recently developed surface modification technique, free amino groups have been introduced onto polyester-type polyurethane (PU) scaffolds.
Methyl-methane-thiosulfate-labeled cysteine and iTRAQ modification of free amine in the amino terminus and lysine were set as fixed modification.
The rheological properties of polypropylene (PP) were controlled by long chain branching (LCB) modification with free radical reaction and its crystallinity.
The rheology of polystyrene (PS) was controlled by molecular modification with free radical reaction, and PS with long chain branching (LCB) level ranging from 0.15 to 1.6 branching point per 104 carbon atom was gotten.
This by-product of glycolysis induces rapid and non-enzymatic modification of free amino groups of lysine and arginine residues of proteins, leading to the generation of advanced glycation end products (AGEs) [2].
