Sequence analysis indicates that among these new findings there are two RNA helicases Has1p and Dbp4p, putative RNA modification enzymes Cbf5p (pseudouridylate synthase-like) and Nop1p (methyltransferase-like), as well as nucleolar proteins Nop56p, Nop58p, and Rsa3p.
We obtained DNA restriction and modification enzymes from New England Biolabs Inc. (Beverly, MA, USA) and polymerase chain reaction (PCR) reagents from Promega (Madison, WI, USA).
Restriction and modification enzymes were from New England Biolabs (Ipswich, MA) and all chemicals used were from Sigma-Aldrich (St . Louis MO).
Restriction and modification enzymes were from New England Biolabs (Ipswich, MA, USA).
Arf1, which shares a 34% sequence identity with Arfrp1, plays a general role in regulating membrane recruitment of various vesicle coat proteins and lipid modification enzymes (Donaldson and Jackson, 2011).
All the restriction and modification enzymes used were from New England Biolabs (Hitchin, Herts, UK) or from Roche Diagnostics (Mannheim, Germany).
DNA restriction or modification enzymes were from Fermentas (Vilnius, Lithuania) or New England Biolabs (Ipswich, MA, USA).
Restriction and modification enzymes were purchased from New England Biolabs (Beverly, MA).
Restriction and DNA modification enzymes were purchased from New England Biolabs, Fermentas or TransGen.
Restriction and modification enzymes were purchased from Fermentas, New England Biolabs and Invitrogen.
