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For better prediction performance we introduced ChIP experiment data and histone modification data in constructing our predictor.
Though there are 31,237 distinct CpG loci with high-throughput DNA methylation and chromatin modification data in the CM profile used for model construction and regulatory relationship inference, it is not quite enough for generating robust results.
Here this issue was investigated by using publicly available histone modification data in yeast.
To test this hypothesis we applied the "differential" algorithm (see Formulation) to histone modification data in myoblasts and myotubes.
All of the modification data in this study are normalized to the density of histone H3; therefore, the co-modification might be caused by histone H3.
For ease of model description, we consider the problem of chromatin state segmentation on multi-species histone modification data, in which case multiple conditions correspond to multiple species.
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A study from Xu et al [ 11] employed a Hidden Markov Model (HMM) to identify the differential binding enrichments from two ChIP-seq samples (ChIPDiff), which was tested on three histone modifications data in two different cell types.
Consistently, the 273 genes are preferentially expressed in neurons, as gauged from H3K4me3 modification data collected in neurons and non-neuronal cells in the human PFC [ 33] (Simulation test, p < 0.0001, Figure 4f), and neuron-specific gene expression data collected in the mouse brain [ 34] (Fisher's exact test, p < 0.0001 after Bonferroni correction, Figure 4g, see Methods).
When we examined the arrangement of probes in the chips that provided the modification data used in this study [ 21], we could not find any correlation between the location of probes in chips and their position in the genome.
Taking advantage of the cytosine modification data profiled in the CEU and YRI samples (12), we evaluated if there were population-specific CpG sites located in the MYLK gene.
A full list of the histone modification data sets used in this study can be found in the following file (http://datashare.is.ed.ac.uk/bitstream/handle/10283/239/1756-8935-5-6-s6.xlsx, last accessed July 1 , 2014.
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