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11671_2012_1047_MOESM1_ESM.pdf Additional file 1: Figure S1.Comparison of ITO substrates before and after modification by chloroform.
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The outer membrane of E. coli XL1-Blue was permeabilized by chloroform to expose the peptidoglycan for digestion as described by Lavigne et al. (2004) with some modifications and used as the substrate for peptidase M15A.
The method used methanol: chloroform, followed by chloroform and water extraction.
The liberated atorvastatin (acid form) was extracted by chloroform.
Amplified DNAs were purified by chloroform extraction and ethanol precipitation.
RNA was isolated by chloroform extraction and precipitated using isopropanol.
Samples were extracted with phenol-chloroform followed by chloroform before precipitation with EtOH.
This was followed by chloroform:isoamyl alcohol extraction.
Cuticular waxes (CW) were extracted by chloroform dipping.
Nuclear RNA polymerase I activity was also induced by chloroform.
Alkanes were extracted by chloroform-methanol extraction method [ 46] with modifications.
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modification by gender
modification by sex
modification by time
modification by genotype
modification by plasma
modification by obesity
modification by race
modification by type
modification by use
modification by baseline
modification by education
modification by medication
modification by rurality
modification by cigarette
modification by phosphorylation
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