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By irradiating cells with UV light through a 3 µm microporous membrane to induce localised damage, we demonstrate a marked difference in the kinetics and extent of PCNA modification between cells irradiated in S phase and in G1 phase of the cell cycle.
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Intercellular heterogeneity, i.e., differential DNA modification status between cells in a population, is a major cause of phenotypic heterogeneity in many organisms [76].
In this approach a natural language processing module, MedScan, is used to describe events of regulation, interaction and modification between proteins, cell processes and small molecules and the results are generated in the forms of biological pathways, gene regulation networks and protein interaction maps [ 25].
The fact that there is little evidence that underlying DNA has a substantial affect on histone modifications is perhaps unsurprising; patterns and distributions of histone modifications vary between cell types even when from the same genetic background.
We found no changes in LC3B modification between ACT1 treated cells and R-pep or water treated cells even in the presence of the autophagy inhibitor chloroquine (Additional file 1: Figure S2A).
This slight loss of binding activity to the BP antigen may be due to the differences in antibody assembly and post-translational modification between two host cells (CHO-K1 and hybridoma) during the chimerization process from the original mouse hybridoma BP1 7F7.
The most disparately altered chromatin modification between CLDN3-repressed and CLDN3-expressing cells was the repressive H3K27me3 histone mark, although loss of two other repressive marks, H4K20me3 and H3K9me3, likewise associated with CLDN3 derepression, also independently of altered DNA methylation.
The comparison of metabolic flux distributions between before and after genetic modification of cells yielded information on the mechanism of regulation of metabolic flux in microorganisms.
There was no difference in the level and distribution of the single modification between undifferentiated and differentiated mesenchymal stem cells.
Such synthesis must take into account the dynamic state of protein post-translational modifications and protein protein or protein DNA/RNA interactions, cross-talk between signal pathways, feedback regulation within cells, between cells, and between tissues.
Such synthesis must take into account the dynamic state of protein post-translational modifications; protein-protein or protein-DNA/RNA interactions; cross-talk between signal pathways; and feedback regulation within cells, between cells, and between tissues.
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