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A detailed protocol is described in the user's guide kit and was used without modification as reported previously [ 24].
Accordingly, the results described here, and summarized in Tables 1 and 2, are not exhaustive, but represent effect modification as reported by the authors.
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For miRNA gel blot analysis, total RNA samples were extracted from plant materials using RNAiso Plus (Takara), with a few modifications as reported previously (Jung et al. [2007]).
Interestingly, nNOS appears to run lower on the gel in patients with RA compared with healthy controls, which could be due to post-translational modifications as reported previously.
The media used for plant induction, multiplication and growth of the plants was Murashige and Skoog [ 78], at pH 5.7, with some modifications as reported by Robert et al. [ 16, 55].
The total antioxidant capacity of the rooibos extract was assessed by measuring the oxygen radical absorbance capacity (ORAC), trolox equivalent antioxidant capacity (TEAC) and ferric ion reducing antioxidant power (FRAP) according to methods described by Ou et al. [ 19], Re et al. [ 20] and Benzie and Strain [ 21] respectively with some modifications as reported previously [ 9].
Another advantage of using pyrrolidine compounds as potential anti-MRSA agent is that most of the studies only involve modifications of the pyrrolidine functional group on the chemical structures of well-known wide-spectrum antibiotics such as fluoroquinolones and carbapenems [ 42– 45] rather than modification on the pyrrolidine structure itself as reported previously [ 46].
Alternatively, GADD45γ may function to activate MAP3K4 in pathways unrelated to DNA methylation, perhaps involving other epigenetic modifications, as reported in stem cell contexts (Abell et al., 2011).
177Lu-DOTATATE/-TOC was prepared with minor modifications as previously reported [14],[14].
Sample preparation was carried out with modifications as previously reported (Atherton et al. 2006; Moazzami et al. 2011).
Cells were prepared following the protocol by Sana et al., with minor modifications as previously reported.
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