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Our analyses question the taxonomic utility of trophic characters (e.g., teeth shape or jaw modification), as previously done by Rosen [ 47] on the basis of intermediate morphological states between Astyanax fasciatus and Bramocharax baileyi.
In mammalian cells HSL is regulated by several kinases and as a result HSL activity undergoes significant modification, as previously shown for several tissues [12], [29], [30].
DNA sequencing was performed after bisulfite modification, as previously described [ 36].
Moreover, recent studies have demonstrated that epigenetic silencing of alleles that occurs during meiosis does not completely erase all epigenetic modification as previously thought (36, 48).
Microarray hybridization and processing, and the image scanning were performed according to the manufacture's recommended protocol (Affymetrix Inc., Santa Clara, CA) using a GenChip resequencing assay kit (Affymetrix) with modification as previously described [ 10].
Genomic DNA (1 μg) from each sample was modified by sodium bisulfite as described by Herman et al (1996) with slight modification as previously reported (Sriraksa et al, 2010).
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177Lu-DOTATATE/-TOC was prepared with minor modifications as previously reported [14],[14].
The ASCs were prepared according to the method developed by Zuk et al. [19] with modifications as previously described [17].
Sample preparation was carried out with modifications as previously reported (Atherton et al. 2006; Moazzami et al. 2011).
cRNA synthesis was carried out using the Affymetrix protocol with minor modifications as previously described [40].
MHC class I tetramers were constructed with minor modifications as previously described [64], [65].
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