The neutral red retention (NR) assay was performed according to Borenfreund and Puerner[86] with slight modifications as detailed in Heger et al.[87] by using RTL-W1 cells.
The preparation of hippocampal cultures was performed essentially as described by Goslin et al. [48] with some modifications as detailed in Dresbach et al. [49].
This assay was performed according to the method of Borenfreund and Puerner (1985) with modifications as detailed: a 0.4%.
E. coli ribosomes were purified and assays were performed essentially as described by Tate and Caskey (1990) with modifications as detailed in reference Soleimanpour-Lichaei et al (2007).
The preparation of hippocampal cultures was performed essentially as described by Goslin and Banker (1991), with some modifications as detailed in Dresbach et al (2003).
We carried out sequencing data evaluation, somatic mutation calling, and additional bioinformatics analysis as described by a pipeline described in [ 8, 9], with minor modifications as detailed in Additional file 4: Figure S3.
The surface preparation, acceptor vesicle immobilization, and donor vesicle incubation were performed essentially as previously described (Kyoung et al., 2011; Kyoung et al., 2012) with modifications as detailed here.
Total genomic DNA was extracted from tissue samples of the foot preserved in 100% isopropanol following the protocol proposed by Sokolov [ 42] with slight modifications as detailed in Sauer & Hausdorf [ 22].
