Sentence examples for modification and sequencing from inspiring English sources

The combination of bisulfite modification and sequencing is the method of choice for methylation mapping in the Epigenome Project [ 27, 28].

In order to assess the degree of xaf1 promoter methylation within these cell lines, a bisulphite DNA modification and sequencing was performed.

A continuous five day treatment with IFN-β caused a significant demethylation of most previously methylated CpG sites that was confirmed by bisulfite DNA modification and sequencing analysis.

We used the conventional bisulfite DNA modification and sequencing method to determine the methylation status in the CpG sites of xaf1 promoter in glioblastoma (SF539, SF295), neuroblastoma (SK-N-AS) and cervical carcinoma (HeLa) cells.

Cells were then grown in these concentrations with daily change of media with fresh DAC for 10 days before DNA was extracted and subjected to bi-sulphite modification and sequencing.

We were concerned about finding an appropriate fragmentation technique that would give us a complete ion series covering the entire tail sequence so that we could confirm sites of modification and sequence variation.

Various changes were made with consensus to the questionnaire (item inclusion, retraction, modification, and sequence) to reduce ambiguity and to make the MLCDP simpler, more user friendly and easier to understand.

Sequencing libraries were prepared using the ChIP-seq DNA sample kit (Illumina, IP-102-1001) with some modifications and sequenced with a Genome Analyzer II (Illumina).

Probe/target pair melting temperatures were balanced by chemical modifications and sequence length adjustment to the coding segments of the probes.

Understanding turnover in the context of histone modifications and sequence variants could provide valuable additional insight into the function of histone replacement.

Quantitative proteomics provides complementary insight to previous work aimed at quantitatively measuring histone turnover, and our results suggest that turnover rates are dependent upon site-specific post-translational modifications and sequence variants.