Interesting questions remain both for understanding the effects of modifications and editing on microRNAs and the interactions between microRNAs and other cellular RNAs such as long noncoding RNAs.
The combinatorial utilization of flexible epigenomic modifications, together with gene splicing, post-translation protein modification and RNA editing has ample potential to generate functional diversity from a fixed genotype.
Taking advantage of ΦC31 attP sites, gene trap cassettes in MiMIC can be easily swapped with any DNA sequence flanked with attB sites by ΦC31 recombinase, allowing further gene modifications and genome editing [ 33].
Recent literature on RNA sequencing highlights several posttranscriptional modifications and RNA editing in many organisms, including M. oryzae, resulting in imperfect alignment of RNAs to the reference genome [ 25, 30].
No alteration in the editing reaction was observed after celecoxib treatment, indicating that editing levels are extremely conserved and editing modifications may occur only after a longer treatment or in a situation of life-time inhibition of COX-2 activity as observed in COX-2−/− mice.
EAM, BHF and CTN: manuscript review, modification, editing, and revision.
From these single base allelic ratios we can readily detect instances of RNA editing, as one type of post-transcriptional modification, and survey the variability of editing at single sites and the extent to which they are edited across different brain regions and across different individuals in the DLPFC (Table 4).
In vascular plants, accumulation of chloroplast transcripts is usually regulated at a post-transcriptional level, such as stability, modification, editing, and translation (reviewed in).
Our results indicate that the modulation and modifications of microRNAs may be context-dependent or specific to experimental conditions; linking together editing, differential expression, modification, and secondary structure, helping us gain insight into the intricate relationships between these events.
Permitted modifications consist of changing the energy coupling, editing the protein annotation and editing the reaction (including the primary substrate and compartment assignments).
