As the expressed partial S proteins contained four N-linked glycosylation sites for both type I (551-NVT-553, 559-NDT-561, 591-NGS-593 and 596-NVT-598) and type II (520-NKS-522, 536-NIT-539, 558-NIT-560 and 566-NNT-568) FCoV, the baculovirus expression system was chosen to provide post-translational modification and complex folding, including glycosylation [ 18],[ 19].
To enhance catalytic performance and stability, numerous methods such as structural modifications and complex formations with other functional materials are proposed, and they are basically based on well-defined and well-ordered catalytic active sites by exquisite control at nanoscale.
For example, in vitro nitration of lysozyme and cytochrome c using peroxynitrite results in combinations of mono-, bis-, and tris-nitration, among other PTM modifications, and complex separation of products is necessary.
Decades of molecular biology research have elucidated a substantial portion of this network, which is currently being reconstructed at a high level of detail that captures cellular processes such as transcriptional regulation, post-translational modification, transport, and complex formation in the formal and computable BioPAX format [ 3].
Overall, the ModCell™ model system provides coverage of more than 620 genes, giving rise to 3397 components (genes, transcripts, proteins, protein modifications, and complexes) that are connected by 5456 reactions.
The possibility of introducing various functions to SWCNT peptide was also demonstrated by several methods, such as introduction of special amino acids, chemical modification, and additional complex formation based on electrostatic interaction.
We measured histone modification and RNAPII complex levels during serum stimulation in a ChIP assay (Fig. 5).
In addition, miR-26a might target genes involved in the regulation of transport, DNA damage response, protein modification and protein complex assembly, as well as nucleotide biosynthesis and RNA splicing.
DOI: http://dx.doi.org/10.7554/eLife.01287.016 GO enrichment analysis for the two sets of differentially retained introns showed distinct gene function enrichment (e.g., protein kinase activity and intracellular targeting in the filopodial stage, and histone modification and myosin complex in the cystic stage), implying that regulated intron retention plays different roles at these stages.
The parameters n j represent the rate constants of transcription and stomata opening, m j are the rate constants of degradation; p j are constants of translation, protein modification and protein complex formation; g j are Michaelis-Menten constants and a, b, c, d, e,f, g, h, i, j are Hill coefficients; q j are the rate constants of acute (P-dependent) light activation of transcription.
More precisely, entity names are combined with other features such as modifications, compartment and complex components.
