The sum index was obtained by totaling the intensity and percentage scores, as follows:, and indicated sum-indexes of 0-1, 2-3, 4-5, and 6-7, respectively; and were defined as no or modest expression, and and were defined as strong expression.
The gene expression pattern of inflammatory molecules in response to PRP showed absence of IL-1b and COX1 and modest expression of IL6, IL8, COX2, and TGF-b1.
The synthesis of IL6 and IL8 proteins induced by PRP is decreased significantly in the presence of hyperuricemia (P = 0.017 and P = 0.012, resp .. Concerning extracellular matrix, PRP-treated tendon cells displayed high type-1 collagen, moderate type-3 collagen, decorin, and hyaluronan synthase-2 expression and modest expression of scleraxis.
Since we observed robust expression of SOX2 and modest expression of c-MYC and KLF4 in our human NSC cultures, we tested if ectopic expression of OCT3/4 per se would be sufficient to convert them from multipotent to pluripotent stem cells.
Small-scale experiments (e.g., 1/16 – 1 plate) provide a wealth of information including the tagging of many or most expressed genes, microsatellite markers, full-length sequencing of highly expressed genes, and modest expression level information.
In contrast to the modest expression of P-EphA2S897 and LRP1 in GBM tissues, nominal expression was noted in normal brain tissue (panels m and o), and EphA2 expression was similarly weak (panel n).
Among the six Slitrk family genes (Slitrk1 6) in mice, Slitrk6 shows a unique expression pattern, with strong expression in the inner ear and modest expression in the dorsal thalamus at both embryonic and postnatal stages [1], [4] [6].
At day 6, Act A-LC presented an immature phenotype with a modest expression of CD80, CCR7 and CD83 (Fig. 1A).
In Nodal-lacZ heterozygous embryos of 5 12 somites, β galactosidase staining reported robust Nodal expression around the node and in the left LPM of wild type embryos (n = 32) whereas compound Nodal-lacZ heterozygous, Tbx6 mutant embryos equivalent to 3 10 somite stages as judged by head morphology showed only modest expression around the node and no expression in the LPM (n = 8)(Figure 2E,F).
Based on previous studies using these promoters in the eye, we initially hypothesized that the IRBP promoter would drive modest expression selectively in rods and cones [33], while the CBA promoter would direct high expression levels in multiple ocular cell types.
Modest expression levels of LRFN4 and LRRN2 were observed but only in EBs.
