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At a modest depth of sequencing, viral transcripts can be detected at frequencies less than 1 in 1,000,000.
Thus, since budget is often a concern, it is recommended to increase the number of samples, which correspond to biological replicates, sequenced at a modest depth, rather than to increase sequencing depth in fewer samples.
We observed that all high confidence CDR3βs identified by RNA-seq (n = 9) fell within the top 2.1 % (n = 60) of CDR3βs detected by TCR-seq, ranked by abundance (data not shown), confirming that at a modest depth of sequencing, RNA-seq can identify the most abundant CDR3-encoding transcripts.
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Trump seems to believe that foreign affairs require only modest depths of thought.
Vladimir Putin and Recep Tayyip Erdoğan Trump seems to believe that foreign affairs require only modest depths of thought.
Since modest depths of shielding stop the vast majority of Solar Energetic Particles (SEP), the greater challenge is posed by the need to shield the crew from the Galactic Cosmic Rays (GCR), which include highly-charged and highly-energetic particles.
For example, the ENCODE RNA-seq standards [ 20] suggest that transcriptome profiling of polyadenylated positive samples require modest depths of sequencing, such as 30 million PE reads of length >30 bp, while for novel transcript discovery and strong quantification of known transcript isoforms deeper sequencing is required.
The Angels bought some modest outfield depth on Wednesday, acquiring Kirk Nieuwenhuis from the New York Mets for cash.
Metagenomics alone, at a modest sequencing depth, cannot provide a highly resolved view of the community structure because of its bias toward sequences of the most abundant taxa.
Our chosen sequencing platform does not lend itself to the evaluation of copy number in these tumours, owing to the comparatively modest read depth.
Thus, power to detect heterozygosity within a given genomic window is fairly good because of the large number of diagnostic SNPs in each window, even though detecting heterozygosity at any individual SNP is low, given the modest sequencing depth.
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CEO of Professional Science Editing for Scientists @ prosciediting.com