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In both recognition- and reactivity-based modes, the probe provides information based on a chemical interaction rather than on an intrinsic property of the metal.
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In the first mode, the probe can be operated selecting a combination of excitation traces to drive and sensing coils to read while is being moved on the tested part.
In this mode, the probe is oscillated at a low frequency (1 2 kHz), capturing a force curve each time the AFM tip taps on the sample's surface.
In this mode, the probe is oscillated at a low frequency (0.5 kHz), capturing a force curve each time the AFM tip taps on the sample's surface.
The design of SNP GeneChips thus enables us to study how these interaction modes affect the probe intensities in a systematic way.
In this paper, we study the dynamic modes of a scanning near-field optical microscope (SNOM) which uses an optical fiber probe; and the sensitivity of flexural and axial vibration modes for the probe were derived and the closed-form expressions were obtained.
In the cross-allelic hybridization mode (C-mode) the probes bind targets of the alternative allele in duplexes of the type P-AB and P-BA, respectively.
In the allele-specific hybridization mode (called S-mode) the probes bind the target which they intend to detect via duplex formation of the type P-AA and P-BB, respectively.
In the S-hybridization mode the probes bind the aRNA fragments of complementary sequence transcribed from mRNA transcripts which they intend to detect.
In the N-hybridization mode the probes bind aRNA fragments of partly complementary sequence originating however from mRNA transcripts not referring to the interrogated gene.
For tapping mode probes, the probe tip apex achieved a 10 nm radius of curvature without additional sharpening steps; tilt-compensated probes were also fabricated for better scanning performance.
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