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The symmetric increasing and decreasing gradients in the larger region between ori-beta and ori-alpha very well fits with in vivo evidence for clockwise and counter-clockwise modes of replication from ori-beta and ori-alpha, respectively, known for the highly replicative R-plasmid (R6K) and its derivatives in E.coli, carrying a similar arrangement of vegetative replication origins [ 48].
Both of these modes of replication have found uses in biotechnology.
gp32 is therefore important in both modes of replication.
Case study research designs aiming to test theories have to outline modes of replication and the elimination of rival explanations.
RNA viruses can be placed into four different groups depending on their modes of replication.
We have already finished sequencing the whole genome of the strain (unpublished), and found that this strain contains 11 endogenous plasmids with vary sizes and modes of replication and its chromosome consists of 5,401,320 bp.
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The native plasmid, pMSR0 (6823 bp), has 9 ORFs, and replicates by rolling-circle mode of replication.
Eventually, it is likely that the classification of viruses will be based on their nucleotide sequences and their mode of replication rather than on structural components, as is now the case.
Lentiviruses, represented by the human immunodeficiency virus type 1 (HIV-1) are retroviruses that possess complex genomes and a finely regulated mode of replication.
This is precisely what is expected by the semiconservative mode of replication, in which the original DNA molecules should separate into two template strands containing 15N, and the newly aligned nucleotides should all contain 14N.
Such partitioning may be critical for these large and complex phages to maintain their flexibility, while simultaneously allowing them to conserve their highly successful virion design and mode of replication.
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