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Although we agree that this supports our conclusions, the focus of this work is to elucidate the coexistence of different modes of nuclear diffusion in the nucleus, and the consequences for biochemical rates.
We are not the first to show that aPKC is present in the nucleus but our data suggests that there are multiple modes of nuclear transport that differ between aPKC isoforms.
Our results indicate that there are different modes of nuclear migration depending on the phase of the cell cycle; active migration occurs during G2-phase, and passive migration during G1-phase.
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Whilst a nuclear localization signal (NLS) has been shown to mediate nuclear translocation, the mode of nuclear transport remains to be elucidated.
Based on the quantified p53 fluorescent trajectories, we classified the dynamic mode of nuclear p53 manually by eye.
FDR and SDR depend upon the mode of nuclear restitution [ 12, 15] and result from the omission of the first or the second meiotic division, respectively.
More importantly, the mode of nuclear motion of molecules has physiological implications as it affects how proteins find their target sequences in the genome, how RNAs are exported from the nucleus upon transcription and how proteins are sequestered within the nucleus for regulatory purposes.
Deciphering whether E75 and UNF heterodimerize or bind to adjacent sequences, how they cooperate with CLK/CYC, and whether any ligand is involved in their transcriptional regulation will yield new insights into the diverse mode of nuclear receptor crosstalk and their critical roles in circadian biology.
In this study, the nuclear level density parameters of some deformed radioisotopes of target nuclei (W, Hg) used on the accelerator-driven subcritical systems (ADS) have been calculated taking into consideration different collective excitation modes of observed nuclear spectra near the neutron binding energy.
To further characterize the mode of PIR nuclear translocation upon proteasome inhibition, we have considered the possibility that proteasome-sensitive p53 binding proteins, are responsible for carrying PIR into the nucleus.
The origin of the soluble form of nTRAIL-R2 as well as the mode of its nuclear translocation is currently completely unknown.
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