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For non-covalent interactions, generally there are three modes for binding viz.
Cryo-electron microscopy structures of SRP-ribosome complexes [29], [30] have revealed different modes for binding signal sequences in SRP bound to a ribosome-nascent chain complex.
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However, it is unclear whether this is a general mode for binding unstructured RNA in archaeal Cas6 homologues.
Although mutations of this Pro has shown that it is not necessary for the cluster arrangement, it provides an optimal environment for the next cluster by both providing hydrophobicity and supporting a specific turn mode for binding.
The functional evidence presented above, combined with structural information for thrombin·ornithodorin [25] and thrombin(E192Q)·BPTI complexes [23], immediately suggested two possible modes for boophilin binding to cognate thrombin (Figure 4A).
In recent studies on Pol X−DNA interaction, it was proposed that the enzyme has two distinct modes for DNA binding (28).
By comparing the effect of long and short chains of PPS on the affinity of ADAMTS5-2 and ADAMTS5-5 for TIMP-3, we propose three modes of binding for PPS.
Using the standard staining reagents and protocol [1∶1 dominant binding mode for Ab/streptavidin binding to ligand] [24], single QD fluorescence dots would be considered one or more than one CD3, CD4 or CD8 molecules.
During recent years, a growing number of non-classical modes of binding for CaM towards its target peptides have been detected [1], [7] [11].
This detailed analysis enabled us to define different modes of binding for some of the targets and hence their prospective regulation modes (mRNA cleavage versus translational suppression).
The probable reason for different modes of binding of vesicle-associated membrane protein to botulinum neurotoxin type B and the tetanus toxin is discussed.
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