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Although evaluation of proteomic changes after miRNA addition or deletion provides evidence that tools stringently requiring seed pairing perform better than those tools with more moderately stringent cutoffs [42], [43], perfect base pair matching does not guarantee interaction between specific miRNAs and target genes [35].
Comparisons with TargetScan and EIMMo require stringent seed pairing, while miRBase requires moderately stringent seed pairing.
Sequences were assembled with DNASTAR using moderately stringent parameters (i.e., match size was chosen to be at least 40 nucleotides, and match percentage was 90).
Substitution models for DNA evolution were selected using AIC in ModelTest3.7/ModelTestJ [69]; GTR+G (for original Alignment 1) and GTR+G+I (and moderately stringent Alignment 2 and the stringent Alignment 3).
A moderately stringent process (Alignment 2) eliminated variable sites and retaining 1,756 (64% of the original 2,711 positions; 769 parsimony informative sites) and a stringent process (gblocks: Alignment 3) retained 1,206 highly conservative residues (44% of the original 2,711 positions; 446 parsimony informative sites).
Linear function runs straight with least accommodative power, Gaussian is moderately stringent and Polynomial tries to cover more spread and deviating instances correctly.
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"The quality filter will be pretty stringent.
The genetic maps generated for each of the crosses, and the map that we built based on collinearity information between pearl millet and foxtail millet, were merged using ALLMAPS60 with the most weight assigned to the synteny map followed by the stringent SNP maps, the moderately filtered SNP maps, and finally the GBS sequencing tags (Supplementary Code 3).
While this stringent cut-off undoubtedly eliminated moderately-conserved or clade-specific sequences, it guaranteed the significance of identified positions.
We did two sensitivity analyses defining relapse with more stringent criteria: two or more psychotic symptoms above the threshold, and requiring a score of 5 (moderately severe) to define relapse.
Moderately heavy.
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CEO of Professional Science Editing for Scientists @ prosciediting.com