Exact(3)
Mouse anti-FLAG M2 antibody diluted 1000-fold with Can Get Signal Solution I (TOYOBO, Co. Ltd., Tokyo) was added into each well and the plates were incubated at room temperature with moderately stirring in a 96 well plate shaker (BioShaker V·BR-36, TAITEC, Saitama, Japan) for 1 hour.
After wells were washed with PBST four times, HRP-conjugated anti-mouse IgG antibody diluted 5000-fold with PBST was added into each well and the plates were incubated at room temperature with moderately stirring in a 96 well plate shaker (BioShaker) for 1 hour.
This offering, apart from a moderately stirring finale, was mostly abject.
Similar(55)
Place the lid on the saucepan and cook the contents for 10 minutes, stirring occasionally, until the sugar completely dissolves and a moderately thick syrup forms.
No stirring.
Bleakly stirring.
Thirsty stirring.
Nervously stirring.
Stirring words.
Stirring spoon.
Opponents are stirring.
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