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Within the brain, ASXL1 has much higher expression in the white matter, whereas ASXL3 has moderately higher expression in the insula, cingulate gyrus, and amygdala, with approximately similar expression elsewhere [ 16] (see Additional file 5, Figure S3).
Lines C and D had moderately higher expression, with UCP3 levels of 155 ± 47 (n = 2) and 194 ± 37 (n = 4) ng UCP3/mg total mitochondrial protein, respectively, comparable to those in mouse and rat.
As can be seen in Figure 2, RA patients carrying two copies of the SE had moderately higher expression of both PTPN22_v4 (P = 0.04) and PTPN22-long forms (P = 0.02) compared to individuals with no or only one copy.
This phenotypic category is dominated by lethal alleles, which suggests that female-biased genes (at least those with moderately higher expression in females than males) have a higher proportion of essential functions than male-biased genes.
For the unigenes tested only two showed some discrepancies although both were similarly up-regulated i.e Unigene58054_All showed a much higher expression level while Unigene1735_All had moderately higher expression in qPCR as compared to the RNA-seq results.
In the SAS cells, Oct3/4, Nanog, and ABCG2 genes in the SP cells showed moderately higher expression than those in the MP cells (1.5 2.0-fold 1.5 2.0-foldhereas no dincreasee whereaserved betweenoSP andifference in their expression of Sox2, Notch1, and Bmi-1 genes (Fig. 2a and b).
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All plasmids had the PHO5 promoter for moderately high expression, except Y2S2LO had the weakly expressing YSP2 promoter.
All 20 22 nt Pst-sRNA sequences with moderately high expression levels (>30 total reads) were found in both IP and IL.
Sixteen miRNAs with moderately high expression levels were selected for testing and an additional six miRNAs were used as input normalizers (Additional file 5: Table S5).
Calb3 is known to be a gene transcript that shows a moderately high expression in control samples and is stimulated to high expression levels with high doses of EE as shown by Affymetrix GeneChip data (4,700 units in control to 49,400 units in high-dose group with a chip scaling factor of 1,500).
In contrast, two lines with up-regulation of the XBP1, but no ERLIN2 gene amplification, had moderately high-expression of the ERLIN2 protein.
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