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The polymerization proceeded well in homogeneous manner to give a moderate yield of polymer.
After screening the reaction conditions (Supplementary Tables 1 and 2), a moderate yield of 3a was obtained in the absence of an exogenous ligand (entry 1, Table 1).
Control experiments have demonstrated that both the nickel catalyst and Mn dust are essential for the desired transformation to proceed, while moderate yield of 3 can be obtained in the absence of ligand, indicating the chelating ability of the pendant ester group (See Supplementary Table 1).
In the case of cyclohexene oxide, a moderate yield of 56 % with 63 % selectivity was obtained.
The main advantage of this synthesis is the higher yield of ligand precursors and a moderate yield of complex 64.
Next, we investigated the reaction with 20 mol % of SnCl2·2H2O, where the moderate yield of the product was observed (Table 1, Entry 6).
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Chloroacetone gave moderate yields of the α,β-unsaturated coupling product.
Since a contaminant protein with ATPase activity bound to rOPA1, we removed it by extensive washes with ATP, before we eluted by increasing imidazole concentrations obtaining moderate yields of rOPA1 devoid of the contaminating ATPase activity (Supplementary Fig. 6b).
In the two-phase medium mainly two addition products (in moderate yields) of SCN/NCS (major) and two SCN/SCN groups were formed.
At 30°C, moderate yields of benzil were observed in all three solvents.
In spite of obtaining good to moderate yields of products in our earlier works [21, 22], we obtained higher yields using nano Preyssler catalyst (Table 1).
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