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Hybridization was conducted at moderate stringency with a 300 base pair homologous A. thaliana cDNA probe corresponding to subunit A of the vacuolar type ATPase.
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With moderate stringency statistical thresholds (M-value > 0.5 or < −0.5, p-value < 0.05), 9120 genes were differentially expressed: approximately 65% of the genes in the Drosophila genome.
With a moderate stringency screen (RPMK > 3, P < 0.05, FC > 2), there were 355 up-regulated (Additional file 14: Table S14) and 348 down-regulated genes in the Cd2ap mutant endothelial cells (Additional file 15: Table S15).
The array design (Agilent SureDesign) had baseline 2× coverage, moderate stringency, moderate masking of repeats, and manual boosting in order to reduce replication of high GC-content baits.
The ExCopyDepth count (711) that falls away from the extreme ends of the exCNV count range (47 1537) exhibits the moderate stringency in CNV calling of ExCopyDepth.
For instance, at moderate stringency (TST-adjusted p ≤ 0.01), 25,923 genes (91.4% of all detectable genes) showed significant expression differences among the three soybean lines.
DIG-labeled probe was hybridized in DIG Easy Hyb buffer (Roche) overnight at 50°C and washed at moderate stringency according to the manufacturer's instructions.
Similarly, the PPR-like 1 gene aligns to a portion of PPR-like 2. All of these alignments were using the "moderate" stringency function of GeneSeqer.
Neuroticism, on the other hand, had a moderate positive correlation with examiner stringency, with a correlation coefficient of 0.73 (p = 0.01).
He proved that it could fuse historical stringency with Cold War paranoia; could provide an arena for our techno-dreams and urban myths.
We thus define a set 2359 high stringency sites with t = 16 and a set of 7444 lower stringency with 10
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