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Tumor cells that did not stain for RON expression were scored as negative, cells that weakly stained were scored as 1, moderate staining was scored with a 2, and strongly staining samples were scored as 3. Of the positive tumor cells detected,< 5% were negative, between 5 and 24% were scored as 1, 25 49% were scored with a 2, and > 50% were scored with a 3.

Weak to moderate staining was detected in the adrenal medulla (Figure 3H).

Moderate staining was observed in 8 (6.1%) and strong staining in 9 (6.8%) of cases.

In contrast, no strong or moderate staining was observed in the 17 normal breast tissues.

Four of these showed weak staining and moderate staining was observed in three cases.

Slightly rougher surface with moderate staining was observed in very few cases of all the three materials after 36 months.

However, moderate staining is detected in the majority of ovarian, lymphoma, skin, testicular, uterine, prostate, and adrenal cancers (Zhang et al., 2012b).

Tumors with more than 10% of tumor cells or stromal area showing strong to moderate staining are scored as positive.

Cores with over 10% of strong membrane staining were assigned 3+; cores with over 10% moderate staining were assigned 2+; and cores with over 10% of cells with incomplete membrane staining were assigned 1+.

YKL-40 staining was evaluated as sum of two scores based on percent and intensity of positive staining cells as following (1) percent: no staining is 0 points, <10% of cells stained is 1 point, 11 50% of cells stained is 2 points, and >50% of cells stained is 3 points; (2) intensity: no staining is 0 points, weak staining is 1 point, moderate staining is 2 points, and strong staining is 3 points.

Moderate stain was seen in some larger vascular profiles (but not microvasculature), as well as many (possibly oligodendroglial) cells in the corpus callosum, hippocampal fimbria, and other white matter structures.