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In the cochleae exposed to dH2O, the inner hair cells and pillar cells of Corti's organ showed moderate staining for CD68, while the outer hair cells and Deiters' cells demonstrated extremely weak staining for CD68 (Fig. 1h).

In the cochleae exposed to dH2O, the Deiters' cells of Corti's organ showed intensive staining for MCP1, while the inner hair cells and inner pillar cells exhibited moderate staining for MCP1 (Fig. 3h).

The spiral ganglion cells and inner pillar cells of Corti's organ exhibited moderate staining for myosin light chain (Additional file 5: Figure S5D and S5F), while the hair cells, outer pillar cells, and Deiters' cells displayed mild staining for myosin light chain (Additional file 5: Figure S5F).

In the cochleae exposed to dH2O, the strial intermediate cells, strial basal cells, spiral ganglion cells, and hair cells, pillar cells, and Deiters' cells of Corti's organ showed intensive staining for Rac1 (Additional file 4: Figure S4B, S4D, and S4F), while the spiral ligament fibrocytes (mainly type II) demonstrated moderate staining for Rac1 (Additional file 4: Figure S4B).

A semi-quantitative scoring of the TMA as described earlier revealed weak to moderate staining for aSyn in majority (∼92%) of the benign specimens (n = 25) while a large proportion of (∼84%) of the PDAC specimens (n = 70) exhibited moderate to strong staining patterns (Figure 2F).

Nanoparticle challenge induced moderate staining for 8-OHdG.

- In brief, the Q score is calculated by multiplying the percentage of positive cells (P = 0 for <10%, 1 for 10 25%; 2 for 25 50%; 3 for 50 75%; 4 for >75%) by the intensity (I = 1 for weak staining; 2 for moderate staining; 3 for strong staining), Q = P × I.

The intensity of immunoreactivity was scored as follows: 0 for no staining, 1 for weak staining, 2 for moderate staining, and 3 for strong staining.

For determination of iASPP immunoreactivity, cytosolic staining of yellowish or brownish granules was graded as follows: 0 for background staining, 1 for faint staining, 2 for moderate staining and 3 for strong staining.

The intensity of nuclei staining was reported based on the H-score method using 0 for negative staining, 1+ for weak staining, 2+ for moderate staining, and 3+ for strong staining.

To determine the immunoreactivity of STK33, cytosolic or nuclear staining of the yellowish or brownish granules were graded using the following scale: 0 for background staining, 1 for faint staining, 2 for moderate staining, and 3 for strong staining.