The pattern of staining was variable, and ranged within individual sections from uniform and strong, to a more patchy distribution with weak or moderate staining which did not reach the intensity seen in normal tissues.
Although mild-to-moderate corneal staining does not always require cessation of daytime GP lens wear, it is strongly indicated to discontinue overnight OrthoK treatment temporarily if persistent central corneal staining worse than grade 2 (Efron scale) is observed, to avoid more serious complications such as deep corneal abrasion and/or corneal ulcer.
The average intensity and localization of staining did not differ significantly from normal in hyperplasias or in mild to moderate dysplasias.
In normal liver, bile ducts did not stain for LRG1, whereas hepatocytes showed weak to moderate staining.
HER2+ tumor tissues did not show strong staining with HCAb2 and only 1 of the 4 samples revealed moderate staining with HCAb2.
The immunostaining intensities were categorized by the following scores: 0 (no staining), 1 (weak, but detectable staining), 2 (moderate staining), and 3 (intense staining).
After each staining we evaluated the slides by immunohistochemical reactivity for RANKL using a scoring system of −, +, ++, and +++, which corresponded to absent staining, weak staining (<25 % of cells), moderate staining (<50 % of cells), and strong staining (>50 %), respectively.
Every human tissue specimen was given a score according to the intensity of the nuclear and cytoplasmic staining (no staining = 0; weak staining = 1; moderate staining = 2; strong staining = 3).
Immunohistochemical analysis showed cytoplasmic expression of active caspase 9 with moderate staining intensity.
Score for staining intensity: 0 = negative, 1 = weak staining, 2 = moderate staining, 3 = strong staining.
