Sentence examples for moderate sequencing coverage from inspiring English sources

These pools were subsequently mapped back to their corresponding reference sequences using commonly applied alignment software and at a range of ultra-low to moderate sequencing coverage depths (0.1×, 0.5×, 1×, 2×, 4×, 8× and 16×).

Both of these predicted enzymes are encoded in the Showa transcriptome at low to moderate sequence coverage.

Contigs with low to moderate sequence coverage have been identified for these two inferred enzymes in the B. braunii Showa transcriptome (Additional file 1: Table S15).

All three SSL enzymes are represented by curated contigs of moderate sequence coverage in the Showa transcriptome (Additional file 1: Table S8), with no additional SQS isozymes detectable.

Curated contig 30447 for RPE has moderate sequence coverage at 55.8 reads/kb, while reads for curated contig 32329 for TKTL are highly abundant at 436.6 reads/kb.

Contigs with low to moderate sequence coverage encoding predicted autophagy pathway proteins are present in the Showa transcriptome (Additional file 1: Table S17).

All these contigs have low sequence coverage (less than 25 reads/kb), with the sole exception 09778, which codes for a β-carotene 3-hydroxylase (CrtR, E.C. 1.14.13.129) that has moderate sequence coverage (94.1 reads/kb).

Geranylgeranyl diphosphate (17) is produced by geranylgeranyl diphosphate synthase (GGDPS, E.C. 2.5.1.29), encoded in the Showa transcriptome by only a single nonredundant curated contig with moderate sequence coverage (Additional file 1: Table S7).

A single curated contig with moderate sequence coverage (Additional file 1: Table S9) encodes a putative cycloartenol synthase (CAS, E.C. 5.4.99.8) which may produce cycloartenol ((3 S -2,3-epoxy-2,3-dihydrosqualene, 37), an intermediate in the bioS -2,3-epoxy-2,3-dihydrosqualene

Two nonredundant curated contigs for two putative isozymes of FDPS with 72% amino acid identity were identified in the Showa transcriptome, both with moderate sequence coverage (Additional file 1: Table S7).

A single curated contig with moderate sequence coverage encodes the putative prenyl transferase chlorophyll synthase (ChlG, E.C. 2.5.1.62) that attaches the phytyl side chain onto chlorophyllide-a to yield 48. 47 is also the precursor for the terpenoid side chains of α-tocopherol (vitamin E, 49) and phylloquinone (vitamin K1, 50).