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B. pumilus RecX (BPUM_0795) has moderate sequence conservation with its B. subtilis and B. licheniformis homologs.
The N. crassa dsc- 2 and tul- 1 are possible orthologs of dsc2 and dsc1 in S. pombe, although only moderate sequence conservation was observed (Additional file 3: Figure S2A, B).
Despite moderate sequence conservation between the two orthologs (40%identity/54%% similarity), the two structures are very similar with regard to their overall fold (Cα rmsd of 1.09 Å over 136 atoms).
Given the moderate sequence conservation between the orthologs and differences in the gene repertoire (discussed later), moumouvirus and megavirus clearly are distinct virus species unlike mimivirus and mamavirus that, at >98% mean identity between orthologous proteins and near perfect genomic collinearity, are most appropriately considered strains of the same species (Colson et al. 2011).
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Taken together, the cross-linking data clearly indicate that the topology observed in the CdgS9Output structure represents the biologically relevant model, which is conserved in the two LapD orthologs, LapD and CdgS9, despite only moderate overall sequence conservation (31%identity/52%% similarity).
The roughly constant length of the linker domain in most organisms, together with its moderate level of sequence conservation, suggests that it primarily fulfils a structural role in midasin.
Secondly, besides sequence conservation they depict a conserved secondary structure.
The sequence conservation of KatX1 (BPUM_3712) is moderate, but KatX2 (BPUM_0892) is more diverse, sharing less than 50% identity with B. subtilis and B. licheniformis KatX.
Analysis of sequence conservation identified 354 elements that are conserved.
In addition, circRNAs also exhibit sequence conservation.
Shading indicates levels of sequence conservation (100% conservation: white on black; 75% conservation: black on dark grey).
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