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It was observed that two adenomas were almost entirely necrotic, precluding immunohistochemical categorization and one adenoma, which showed strong diffuse staining for ACTH and GH and moderate scattered staining of LH and TSH, was considered indeterminate by immunohistochemical classification.
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There was scattered staining for Fas throughout the tissues (Fig. 1c,1d).
Normal skin showed abundant staining of the epidermis and there was scattered staining of dermal fibroblasts (Fig. 2a).
However, it was scattered positively stained in both OGE and JE.
The tubular gland cells showed moderate to strong nuclear staining and moderate cytosolic staining.
The above principle has been applied for moderate scattering samples imaged through linear fluorescence microscopy.
Adenomas that showed strong or moderate staining (diffuse, scattered or focal) for one hormone, but no staining for the other five hormones, were classified in the category associated with the expressed hormone (e.g. somatotroph for adenomas that showed positivity only for GH).
Granular, scattered Bax staining was also observed in trophoblast cells in chorion.
Cells were gated according to scatter and staining for CD14 (MDM) or CD3 (T cell).
F480, a macrophage marker, stained scattered macrophages in tumor lesions as expected (Additional file 3).
Scattered foci of staining were observed in the stroma, although nuclear staining was largely absent.
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