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We introduce a full MLE procedure that accounts for sequencing variation, which we show performs better for estimating early events, particularly for samples with moderate read coverage.
We also show that the estimation of the order of events can be sensitive for events that occur early in the progression of the tumor and that the partial maximum likelihood method of Greenman et al. (2012) can give biased estimates, particularly for moderate read coverage or normal contamination.
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A single run of the Illumina MiSeq platform may generate up to 50 million reads, permitting individual sequencing of a whole 384-well plate of BAC clones with moderate depth of read coverage.
We adopted a conservative approach to select genes for SNP analyses based on their RNA-seq coverage, requiring 90% minimum read coverage of each gene length, support for moderate expression level (FPKM > 15) and representation in all three strains.
The obtained read coverage was 96.8-fold.
Average read coverage of coding sequences.
However, read coverage can fluctuate across genomes.
e Mean read coverage per genomic base.
Average read coverage of contigs was 4.5X, and the maximum read coverage was 2,303X.
Read coverage was computed and genome annotation was challenged based on read distribution and coverage.
Larger contig suggests higher sequencing-read coverage.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com