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The 6 selected hits exhibited a strong or moderate MG and BC phenotype, comprised proteins with different C-terminal motifs (see above) and have not been previously characterized.
CG31650, a moderate MG hit with a BC phenotype, is predicted to be a 342 aa soluble ER multiple EF-hand Ca2+-binding protein with a C-terminal SDEL motif.
Furthermore, CG16817, a moderate MG hit, encodes a 184 aa (1 isoform) soluble protein with an extreme C-terminal KKxxx and contains an HSP20-like chaperone domain that binds to and regulates Hsp90 family chaperones in Pichia pastoris.
CG13284, a moderate MG hit with a BC phenotype, contains a C-terminal KKxKxx and is predicted to be a type 1 transmembrane protein with 2 isoforms (325 and 339 aa).
As most of the RNAi depletions leading to BC phenotype displayed also strong or moderate MG phenotypes, we assessed whether there was a causal relationship between the increase in the Golgi number and the increase in cell size.
On the other hand, the moderate MG hit CG13049 is predicted to have 2 isoforms (223 and 181 aa) and 2-6 TMdependingding of the prediction program used).
Similar(51)
The NaCl and B concentrations used in the irrigation water were moderate, 500 mg NaCl L−1and 2 mg B L−1. Sodium chloride and B stress symptoms were recorded using image analysis.
Treatments consisted of different grazing intensities during the winter season: intensive grazing (IG), moderate grazing (MG), and no-grazing (NG).
In this research we conducted a 5-year grazing experiment including 4 different grazing intensities (no grazing, UG; light grazing, LG; moderate grazing, MG; and heavy grazing, HG) in an alpine meadow on the northeastern margin of Qinghai-Tibetan Plateau (QTP).
Calves received moderate growth (MG) or low growth (LG) all-milk dietary treatments, formulated to support daily gains of 0.5 or 0.25 kg/d, respectively, per the dietary energy recommendation for milk-fed calves according to the National Research Council's Nutrient Requirements of Dairy Cattle.
Proliferation of HAO cells at 48 h was stimulated by moderate concentrations (2.5 mg; 5 mg) of OCHS (each p < 0.001), whereas a high concentration (7.5 mg) of OCHS was inhibitory (p = 0.009).
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