Ob-Ra was highly expressed in liver and spleen, whereas moderate expression was observed in heart, testis, and muscle, and its expression was the lowest in lung and kidney.
Moderate expression was demonstrated in non-transformed HCECs, as well as the Lovo and HCT116 cancer cells.
Moderate expression was observed in the rostral NTS and in the nucleus raphé obscurus.
In contrast, ID2 was not expressed in the nuclei of partially or well-differentiated tumors, while weak to moderate expression was observed in the cytoplasm (Figure 1K P).
Moderate expression was observed in the frontal cortex including the PFC (infralimbic and prelimbic), anterior cingulate, MCx, SCx, entorhinal cortex, piriform cortex, orbitofrontal cortex, amygdala, ventral hypothalamus, Purkinje and granule cell layers of the cerebellum.
When NELL1 transcript levels were investigated by RT-PCR in a tissue panel, high expression became apparent in small intestine, kidney, prostate, and brain, whereas moderate expression was seen in colonic mucosa and in immune-relevant organs/cells such as thymus and spleen (Figure 2A).
However, their moderate expression is also observed in the absence of FAs (Schimizu et al. [2013]).
Hence, while pAP42 is useful for strong constitutive expression of target genes, pAP43 may be used if moderate expression is required.
The threshold level for ROS1 positive expression in IHC differs among reports, but 2+ or moderate expression is usually considered to be positive.
In contrast, the euAGL6 lineage has overall retained its expression domain confined to floral tissues and moderate expression is also observed for vegetative tissues of Actinidia.
Immunostaining and Northern blot techniques have revealed an extremely limited distribution in normal tissues, moderate expression being found in gastric mucosa, fetal lung and muscle.
