However, the number of genes with moderate expression values (1 < FPKM ≤100) or high expression values (FPKM >100) in stages MS1-MS4 were increased relative to stages CO or GC, suggesting up-regulation of genes to meet the requirements of MS formation (Table 2).
For clustering, data were filtered to include only genes that showed at least moderate expression (expression value greater than 5) and a range of expression values across all samples (only the top one-third of genes as ranked by the expression range) were used for the clustering analysis.
Lead author Mathew Guest, at Durham University, says: "With the vast majority of Christian students affirming a moderate expression of values in keeping with wider British culture, the Church faces a difficult decision about whether to adapt to changing times or risk permanently alienating an entire generation".
In particular, three samples (2, 6, 10) showed a silent expression of SPARC gene, while samples 1, 3, 5, 7 had a moderate expression of SPARC (value between 1- and 10-fold increase) compared to normal.
Low, moderate and high expression values are calculated on the basis of the microarray clones fluorescence intensity and correspond respectively to values lower than 3,000, comprise between 3,000 and 10,000 and higher than 10,000 arbitrary fluorescence units.
A threshold of 45.8 was defined, representing the mean AURKB expression value, dividing the patients into a group with excessive AURKB overexpression and moderate AURKB overexpression.
However, this value might underestimates the true increase of CB1 receptor in AAV-Stop-CB1 treated pyramidal neurons, given the moderate expression levels of endogenous CB1 protein in this neuronal subtype.
In the analysis of OPG expression, it shows moderate expression at 2, 4 weeks, and decrease at 8 weeks.
Moderate expression of SEC16 generates more ERESs facilitating the formation of COPII vesicles.
However, their moderate expression is also observed in the absence of FAs (Schimizu et al. [2013]).
