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Eight and seven genes displayed strong and moderate expression levels, respectively, and four genes were expressed at a low level (Additional file 4: Table S3).
The vectors/doses were designed for moderate expression levels within neurons.
However, the other three liver-specific miRNAs (miR-148, miR192 and miR-194) exhibited moderate expression levels (Fig. 5).
For subsequent experiments, stable cell lines with moderate expression levels of RC complexes and ATP synthase-FP were selected.
At moderate expression levels, CPY* is efficiently degraded by ERAD, with some molecules packaged into COPII vesicles and retrieved from the Golgi beforehand [16].
Both of these mice had moderate expression levels of hVEGF-A165 in serum after 1 week (240 and 710 pg/ml).
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Both 132PUI and 90PUI plants had a moderate expression level in 1-week-old leaves.
This may reflect the excessive expression levels of the fusion protein, as a moderate expression level is required for retention of the protein in the cytosol [27].
In that study, let-7a, let-7b, miR-21 and miR-27b also showed a moderate expression level [17], which is in line with this study (Fig. 5).
PC3 cells, with a moderate expression level of cystatin C were chosen for subsequent functional studies to evaluate the effects of forced overexpression and knockdown of cystatin C on PC3 cell invasion.
The fraction of GFP-SNX4 that co-localized with CHC at moderate expression level was quantified to be 27.8±6.6% (average with standard deviation, n = 15), and with tubulin 19.4±7.6% (average with standard deviation, n = 5).
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